A STEPWISE METHOD FOR THE ISOLATION OF ENDOTHELIAL CELLS AND SMOOTH MUSCLE CELLS FROM INDIVIDUAL CANINE CORONARY ARTERIES

MICHAEL K. DAME; XINWEN YU; ROSARIO GARRIDO; WALTER BOBROWSKI; J. ERIC MCDUFFIE; HEDWIG S. MURPHY; MUDHER ALBASSAM; JAMES VARANI

doi:10.1290/1543-706X(2003)039<0402:ASMFTI>2.0.CO;2

How to translate text using browser tools

1 November 2003 A STEPWISE METHOD FOR THE ISOLATION OF ENDOTHELIAL CELLS AND SMOOTH MUSCLE CELLS FROM INDIVIDUAL CANINE CORONARY ARTERIES

MICHAEL K. DAME, XINWEN YU, ROSARIO GARRIDO, WALTER BOBROWSKI, J. ERIC MCDUFFIE, HEDWIG S. MURPHY, MUDHER ALBASSAM, JAMES VARANI

Author Affiliations +

In Vitro Cellular & Developmental Biology - Animal, 39(10):402-406 (2003). https://doi.org/10.1290/1543-706X(2003)039<0402:ASMFTI>2.0.CO;2

Abstract

Methods for the stepwise isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries are described. Both cell types can be isolated in pure culture with high yields. Dogs are a common species used in the study of atherosclerosis and coronary artery disease. Capacity to isolate endothelial cells and smooth muscle cells from individual canine coronary arteries should prove useful in the study of coronary artery disease.

Citation Download Citation

MICHAEL K. DAME, XINWEN YU, ROSARIO GARRIDO, WALTER BOBROWSKI, J. ERIC MCDUFFIE, HEDWIG S. MURPHY, MUDHER ALBASSAM, and JAMES VARANI "A STEPWISE METHOD FOR THE ISOLATION OF ENDOTHELIAL CELLS AND SMOOTH MUSCLE CELLS FROM INDIVIDUAL CANINE CORONARY ARTERIES," In Vitro Cellular & Developmental Biology - Animal 39(10), 402-406, (1 November 2003). https://doi.org/10.1290/1543-706X(2003)039<0402:ASMFTI>2.0.CO;2

Received: 5 December 2003; Accepted: 1 December 2003; Published: 1 November 2003

KEYWORDS/PHRASES

PUBLICATION TITLE:

COLLECTION:

PUBLICATION YEARS